Pharmacokinetics and stability of methoxyflavones from Kaempferia parviflora in Thai native roosters
Vibuntita Chankitisakul1,2, Supakorn Authaida1, Wuttigrai Boonkum1,2
1Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Frontiers in Veterinary Science
|April 23, 2025
Summary
Methoxyflavones from Kaempferia parviflora extract were rapidly absorbed in roosters, with optimal extraction using acetonitrile. Stability studies inform dosage and storage for poultry feed applications.
Area of Science:
- Pharmacology and Toxicology
- Animal Nutrition
- Phytochemistry
Background:
- Kaempferia parviflora (KP) is a Thai medicinal plant rich in methoxyflavones.
- Understanding the pharmacokinetics and stability of KP methoxyflavones is crucial for its application in animal feed.
- Thai native roosters serve as a relevant model for poultry studies.
Purpose of the Study:
- To characterize the pharmacokinetics of methoxyflavones from KP extract in Thai native roosters.
- To evaluate the stability of methoxyflavones in blood and plasma samples.
- To determine optimal extraction methods and inform dosage for poultry feed additives.
Main Methods:
- Oral administration of KP ethanolic extract to roosters at varying doses (100, 150, 200 mg/kg).
- Plasma sample preparation using acetonitrile, ethyl acetate, or a mixture to assess extraction efficiency.
- Quantification of methoxyflavones using validated High-Performance Liquid Chromatography (HPLC).
- Pharmacokinetic parameter calculation and stability assessment over seven days at -20°C.
Main Results:
- Methoxyflavones exhibited rapid absorption (Tmax: 1.17-1.83 h) and dose-dependent plasma concentrations (Cmax: 0.34-0.83 µg/mL).
- Slow elimination was observed with half-lives ranging from 2.03 to 2.60 hours.
- Acetonitrile demonstrated superior extraction efficiency for key methoxyflavones (PMF, DMF, TMF).
- High stability (>96%) was maintained for two days, with significant degradation after seven days.
Conclusions:
- KP methoxyflavones are rapidly absorbed and exhibit dose-dependent pharmacokinetics in roosters.
- Acetonitrile is the preferred solvent for methoxyflavone extraction from blood samples.
- Optimal storage conditions are essential to maintain methoxyflavone integrity for poultry feed applications.
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