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Microfluidic Array Enables Rapid Testing of Natural Compounds Against Xylella fastidiosa.

Francesca Costantini1, Erica Cesari1, Nicola Lovecchio2

  • 1Research Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), 00156 Rome, Italy.

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Summary

Natural compounds effectively combat the plant pathogen Xylella fastidiosa (Xf). Microfluidic testing offers a faster, more efficient alternative to traditional methods for evaluating antibacterial susceptibility.

Keywords:
Xylella fastidiosaantibacterial activitybiofilm formationmicrofluidicsplanktonic growth

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Area of Science:

  • Plant Pathology
  • Microbiology
  • Agricultural Science

Background:

  • Xylella fastidiosa (Xf) is a bacterial pathogen causing significant agricultural losses.
  • Its wide host range and challenging management necessitate novel control strategies.
  • Natural compounds are being explored as eco-friendly alternatives for plant disease management.

Purpose of the Study:

  • To evaluate the in vitro antibacterial effects of Trametes versicolor extract, clove essential oil, and Fossil® against Xylella fastidiosa.
  • To compare the efficacy and efficiency of microfluidic-based antibacterial susceptibility testing (AST) with traditional broth macrodilution.
  • To assess the potential of microfluidics for real-time bacterial behavior observation and rapid AST.

Main Methods:

  • Antibacterial susceptibility testing (AST) of Xylella fastidiosa using microfluidic channels.
  • Comparison of microfluidic AST with the traditional broth macrodilution method.
  • Evaluation of minimum inhibitory concentration (MIC) and biofilm inhibition for tested natural compounds.

Main Results:

  • Microfluidic AST provided faster results, reduced material usage, and enabled real-time observation compared to macrodilution.
  • All tested natural compounds (Trametes versicolor extract, clove essential oil, Fossil®) demonstrated high antibacterial efficacy against X. fastidiosa.
  • Comparable results were obtained between the microfluidic and macrodilution methods for antibacterial efficacy.

Conclusions:

  • The tested natural compounds possess significant antimicrobial properties against Xylella fastidiosa.
  • Microfluidic channels represent a promising, efficient, and rapid technique for antibacterial susceptibility testing.
  • This approach can facilitate the selection of eco-friendly products for controlling Xylella fastidiosa in agricultural applications.