Related Experiment Video
Updated: May 8, 2025
Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection
Published on: May 9, 2022
Mouse Model of Brain Tumor Resection
Divsha Sher1, Alina Brosque1, Dinorah Friedmann-Morvinski2,3
1School of Neurobiology, Biochemistry & Biophysics. George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
Abstract:
Glioblastoma is one of the most common and aggressive primary brain tumors, displaying poor survival rates and little success in clinical trials. Following the conventional treatments, which include surgical resection, radiotherapy, and chemotherapy, the average survival time for patients remains low, and even in those who respond to first-line treatments, the tumor eventually recurs. Additionally, due to the sensitive location of these tumors and their infiltrative nature, many patients undergo partial resection, leaving the remaining cells to grow and recolonize the brain. For this reason, models that recapitulate the treatment of patients after surgery have become essential.This protocol describes the resection of tumors in mice following cell injection to the cortex. We recommend that the day of resection be adjusted to the cell line latency and tumor size. This model can be used for assessing the success of different therapeutic strategies and their appropriate timing in combination with surgical resection.
Insights
Glioblastoma tumor resection in mice provides a vital model for testing new treatments. This surgical approach helps evaluate therapeutic strategies following tumor removal, crucial for improving patient outcomes.
Area of Science:
- Neuro-oncology
- Surgical oncology
- Preclinical cancer models
Background:
- Glioblastoma is an aggressive primary brain tumor with poor prognosis and limited treatment success.
- Conventional treatments offer low survival rates, and tumors frequently recur even after initial response.
- Partial resection is common due to tumor location and invasiveness, necessitating models for post-surgical treatment evaluation.
Purpose of the Study:
- To describe a protocol for glioblastoma tumor resection in mice.
- To establish a preclinical model that mimics post-surgical patient treatment scenarios.
- To facilitate the assessment of therapeutic strategies combined with surgical intervention.
Main Methods:
- Protocol details surgical resection of induced glioblastoma tumors in the mouse cortex.
- Recommends adjusting resection timing based on cell line latency and tumor size.
- Utilizes a reproducible surgical technique for consistent model generation.
Main Results:
- The described protocol enables the creation of a relevant glioblastoma surgical resection model in mice.
- This model allows for the evaluation of therapeutic interventions post-resection.
- Timing of resection can be tailored for optimal experimental design.
Conclusions:
- This mouse model is essential for recapitulating post-surgical glioblastoma treatment.
- It serves as a valuable platform for assessing novel therapeutic strategies and their timing relative to surgery.
- The model aids in advancing research for more effective glioblastoma therapies.
