Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbiome of the Eye01:22

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Olink Proteomics Analysis Reveals Aqueous Humor Inflammatory Biomarkers in Patients with Different Stages of Diabetic Retinopathy.

Journal of proteome research·2026
Same author

HSF4 promotes lipid peroxidation by transcriptionally regulating ALOX15 during lens terminal differentiation.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Predicting intradialytic exercise intolerance in maintenance hemodialysis patients: an interpretable machine learning approach integrating functional assessments.

Renal failure·2026
Same author

A Field Emission X-Ray Source Array for Stationary Digital Chest Tomosynthesis Applications.

Sensors (Basel, Switzerland)·2026
Same author

Evolution of overlying strata and disaster-causing mechanisms under inclined thin coal seam group mining: insights from numerical simulations.

Scientific reports·2026
Same author

Targetable driver gene-tumor immune microenvironment axis in non-small cell lung cancer: from molecular pathological mechanisms to precision immunotherapy stratification strategies.

Frontiers in immunology·2026

Related Experiment Video

Updated: Jul 2, 2026

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

18.8K

Macrophage-Hosted Porphyromonas gingivalis Is a Risk Factor for Cataract Development.

Dongzhe Zhang1, Junwei Qu1, Cuncun Ke1

  • 1Division of Vision Science, Joint National Laboratory for Antibody Drug Engineering, Henan University, Kaifeng, China.

Investigative Ophthalmology & Visual Science
|April 23, 2025
PubMed
Summary

Porphyromonas gingivalis (PG) infection is linked to cataract development, particularly in specific types like diabetic and high myopia cataracts. PG accesses lens epithelium via macrophages, promoting cataract progression through oxidative stress and inflammation.

More Related Videos

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
09:52

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

17.5K
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

26.2K

Related Experiment Videos

Last Updated: Jul 2, 2026

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

18.8K
A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
09:52

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

17.5K
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

26.2K

Area of Science:

  • Ophthalmology
  • Microbiology
  • Immunology

Background:

  • Cataracts are a leading cause of vision loss globally.
  • The role of bacterial infections in cataractogenesis remains an area of active investigation.

Purpose of the Study:

  • To investigate the association between Porphyromonas gingivalis (PG) infection and the development of cataracts.
  • To elucidate the mechanisms by which PG may contribute to cataract formation.

Main Methods:

  • Detection of PG 16s ribosomal RNA gene in aqueous humor and anterior capsule tissues using PCR and FISH.
  • Immunofluorescence for RpgA expression and fibrosis markers.
  • Flow cytometry for reactive oxygen species and apoptosis.
  • RNA deep sequencing for differential gene expression analysis.

Main Results:

  • PG DNA was detected in 43.3% of cataract aqueous humor samples, significantly higher than in glaucoma samples.
  • PG was found in 82-94% of cataractous anterior capsule tissues.
  • PG-positive macrophages were identified in the lens epithelium, correlating with increased fibrosis markers and oxidative stress in a rat model.
  • RNA sequencing revealed upregulation of pathways related to epithelial-mesenchymal transition, oxidative stress, and cell death in PG-infected cataract tissues.

Conclusions:

  • Chronic Porphyromonas gingivalis infection can contribute to cataract development by reaching the lens epithelium via macrophages.
  • PG infection promotes cataractogenesis through mechanisms involving oxidative stress, apoptosis, and epithelial-mesenchymal transition.
  • Porphyromonas gingivalis represents a novel risk factor for cataract development.