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Updated: May 12, 2025

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
Published on: March 9, 2022
Fungal Argonaute proteins act in bidirectional cross-kingdom RNA interference during plant infection
An-Po Cheng1, Lihong Huang1, Lorenz Oberkofler1
1Faculty of Biology, Chair of Genetics, Faculty of Biology, Ludwig Maximilians University of Munich, Martinsried 82152, Germany.
Abstract:
Argonaute (AGO) proteins bind to small RNAs to induce RNA interference (RNAi), a conserved gene regulatory mechanism in animal, plant, and fungal kingdoms. Small RNAs of the fungal plant pathogen Botrytis cinerea were previously shown to translocate into plant cells and to bind to the host AGO, which induced cross-kingdom RNAi to promote infection. However, the role of pathogen AGOs during host infection stayed elusive. In this study, we revealed that members of fungal plant pathogen B. cinerea BcAGO family contribute to plant infection. BcAGO1 binds to both fungal and plant small RNAs during infection and acts in bidirectional cross-kingdom RNAi, from fungus to plant and vice versa. BcAGO2 also binds fungal and plant small RNAs but acts independent from BcAGO1 by regulating distinct genes. Nevertheless, BcAGO2 is important for infection, as it is required for effective pathogen small RNA delivery into host cells and fungal induced cross-kingdom RNAi. Providing these mechanistic insights of pathogen AGOs promises to improve RNAi-based crop protection strategies.
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