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Assessing the protective effects of nanoceria on angiotensin II-induced cardiac injury in H9c2 cardiomyoblasts using proteomic analysis

  • 0Proteomics unit, Obesity Research Center, College of Medicine, King Saud University, PO Box 2925, Riyadh 11461, Saudi Arabia.
Journal of Pharmaceutical and Biomedical Analysis +

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April 23, 2025

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April 23, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Cerium oxide nanoparticles (nanoceria) show protective effects against angiotensin II (Ang II) induced cardiovascular damage. Nanoceria pretreatment reversed Ang II-induced protein changes linked to oxidative stress in H9c2 cardiomyoblasts.

Area Of Science

  • Biochemistry
  • Nanomedicine
  • Cardiovascular Research

Background

  • Angiotensin II (Ang II) stimulation causes oxidative stress and inflammation, contributing to cardiovascular diseases.
  • Cerium oxide nanoparticles (nanoceria) possess antioxidant properties and show potential for mitigating Ang II's harmful effects.

Purpose Of The Study

  • To investigate the protective effects of nanoceria against Ang II-induced pathology in H9c2 cardiomyoblasts.
  • To compare proteomic changes induced by Ang II alone versus nanoceria pretreatment followed by Ang II stimulation.

Main Methods

  • Proteomic analysis using 2D-DIGE to visualize differentially expressed proteins.
  • Protein identification via MALDI-TOF mass spectrometry.
  • Statistical analysis including PCA, hierarchical clustering, and canonical pathway analysis.

Main Results

  • 118 differentially expressed protein spots were identified between control, Ang II-treated, and nanoceria-pretreated groups.
  • Ang II upregulated proteins associated with oxidative stress, while nanoceria pretreatment downregulated these and upregulated defense proteins.
  • 18 proteins were downregulated and 35 upregulated in the nanoceria pretreated Ang II group compared to Ang II alone.

Conclusions

  • This study reveals complex proteomic alterations in H9c2 cardiomyoblasts due to Ang II.
  • Nanoceria demonstrate significant protective effects by counteracting Ang II-induced oxidative stress and promoting defense mechanisms.

Keywords: