Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell fate acquisition at a de novo developmental boundary in the maize leaf.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Single-Cell and Spatial Transcriptomic Analysis of Maize Embryo Development: A Sample Preparation Protocol.

Cold Spring Harbor protocols·2025
Same author

Multiplexed transcriptomic analyzes of the plant embryonic hourglass.

Nature communications·2025
Same author

Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.

G3 (Bethesda, Md.)·2024
Same author

Genetic analyses of embryo homology and ontogeny in the model grass Zea mays subsp. mays.

The New phytologist·2024
Same author

NAKED ENDOSPERM1, NAKED ENDOSPERM2, and OPAQUE2 interact to regulate gene networks in maize endosperm development.

The Plant cell·2023
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Jun 17, 2026

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves
08:31

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Published on: December 2, 2016

10.8K

Single-Cell and Spatial Transcriptomic Analysis of Maize Embryo Development.

Hao Wu1, Michael J Scanlon2

  • 1School of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853, USA haowu@njau.edu.cn.

Cold Spring Harbor Protocols
|April 23, 2025
PubMed
Summary
This summary is machine-generated.

This review explores how combining single-cell RNA sequencing and spatial transcriptomics enhances the study of maize (Zea mays) embryo development. These powerful methods offer synergistic insights into gene regulation and developmental processes in plants.

More Related Videos

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.4K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

7.9K

Related Experiment Videos

Last Updated: Jun 17, 2026

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves
08:31

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

Published on: December 2, 2016

10.8K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.4K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

7.9K

Area of Science:

  • Plant developmental biology
  • Genomics
  • Molecular biology

Background:

  • Plant embryogenesis is crucial for development, involving cell division, expansion, and differentiation to form the basic plant body plan.
  • Studying maize (Zea mays) embryogenesis offers insights into fundamental plant development, with potential applications in crop improvement and synthetic biology.
  • Maize embryo development is a complex process regulated by intricate genetic networks operating in specific temporal and spatial patterns.

Purpose of the Study:

  • To review the combined application of single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics for investigating maize embryogenesis.
  • To highlight the synergistic advantages of integrating these two powerful transcriptomic technologies.
  • To discuss how these methods advance our understanding of gene expression and regulatory networks during plant development.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) provides high-resolution gene expression profiles at the individual cell level.
  • Spatial transcriptomics offers transcriptomic data within the spatial context of tissue sections.
  • The review focuses on the integrated use of scRNA-seq and spatial transcriptomics to overcome limitations of each method.

Main Results:

  • scRNA-seq enables detailed cellular profiling but can face challenges in cell cluster identification without known markers.
  • Spatial transcriptomics provides spatial context but typically lacks single-cell resolution and captures fewer transcripts per cell.
  • Combining scRNA-seq and spatial transcriptomics yields synergistic results, offering a more comprehensive understanding of maize embryogenesis.

Conclusions:

  • The integration of scRNA-seq and spatial transcriptomics presents a powerful approach to dissecting complex developmental processes like maize embryogenesis.
  • This combined strategy enhances the ability to identify cell types, understand gene regulatory networks, and map gene expression spatially.
  • Future research can leverage these integrated methods for significant advancements in plant biology, agronomy, and synthetic biology.