An HPLC/push-pull perfusion technique for investigating peptide metabolism

We have combined the push-pull perfusion of radiolabelled peptide, with reverse phase--HPLC procedures as a valuable method for monitoring peptide metabolism in the CNS. This model not only permits studies on metabolism of neuropeptides at specific sites in the CNS, but more importantly, allows (unlike in vitro methods) the study to be made under the appropriate physiological conditions. We have demonstrated unambiguously for the first time, the metabolism of CCK-8 in situ.