Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
Abnormal Proliferation02:23

Abnormal Proliferation

4.3K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
The Ras Gene02:38

The Ras Gene

6.1K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial Intelligence-Enhanced Electrocardiogram for Longitudinal Evaluation of Right Ventricular Dysfunction After Acute Pulmonary Embolism: Rational and Design of CURES-CARE Study.

Pulmonary circulation·2026
Same author

Correction: Global burden and temporal trends of tuberculosis attributable to high sugar-sweetened beverage consumption: insights from the Global Burden of Disease Study 2021.

Frontiers in nutrition·2025
Same author

Global burden and temporal trends of tuberculosis attributable to high sugar-sweetened beverage consumption: insights from the Global Burden of Disease Study 2021.

Frontiers in nutrition·2025
Same author

Dual NIR-II fluorescence and ratiometric photoacoustic imaging-guided metal-phenolic nanosheets for H<sub>2</sub>S-activatable synergistic therapy.

iScience·2025
Same author

Preclinical Evaluation of a Radiolabeled Pan-RAF Inhibitor for RAF-Specific PET/CT Imaging.

Molecular pharmaceutics·2024
Same author

[Retracted] Long non‑coding RNA SLC25A25‑AS1 exhibits oncogenic roles in non‑small cell lung cancer by regulating the microRNA‑195‑5p/ITGA2 axis.

Oncology letters·2024

Related Experiment Video

Updated: May 10, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.5K

Hsa-miR-21 promoted the progression of lung adenocarcinoma by regulating LRIG1 expression.

Li Liu1, Xinhua Liu2, Chengpeng Gao1

  • 1Department of Respiratory, Weifang People's Hospital, No. 151 Guangwen Street, Kuiwen District, Weifang, 261041, Shandong Province, P. R. China.

BMC Pulmonary Medicine
|April 24, 2025
PubMed
Summary
This summary is machine-generated.

MicroRNA-21 (miR-21) may negatively regulate Leucine Rich Repeats and Immunoglobulin Like Domains 1 (LRIG1) in lung adenocarcinoma (LUAD). This interaction impacts LUAD patient prognosis and immune cell infiltration, offering new therapeutic targets.

Keywords:
LRIG1Immune cell infiltrationLung adenocarcinomaPrognosishsa-miR-21

More Related Videos

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

352
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.5K

Related Experiment Videos

Last Updated: May 10, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.5K
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

352
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung adenocarcinoma (LUAD) is a leading cause of cancer mortality with significant molecular heterogeneity.
  • Leucine Rich Repeats and Immunoglobulin Like Domains 1 (LRIG1) expression is decreased in lung cancer and linked to patient outcomes.

Purpose of the Study:

  • To investigate the microRNAs (miRNAs) that target LRIG1.
  • To elucidate the regulatory mechanisms of LRIG1 in LUAD.
  • To explore the relationship between hsa-miR-21, LRIG1, and LUAD progression.

Main Methods:

  • Utilized The Cancer Genome Atlas and Gene Expression Omnibus databases for LUAD patient data.
  • Performed differential expression analysis and gene set enrichment analysis (GSEA).
  • Quantified mRNA and protein expression via RT-qPCR and Western blot; analyzed immune cell infiltration using CIBERSORT.

Main Results:

  • Identified a negative correlation between hsa-miR-21 and LRIG1 expression in LUAD.
  • Observed that high hsa-miR-21 and low LRIG1 expression are associated with poorer LUAD prognosis.
  • Found altered immune cell populations (increased naive B cells, plasma cells, resting CD4+ T cells; decreased regulatory T cells, Macrophages M0) in groups with specific hsa-miR-21/LRIG1 expression patterns.

Conclusions:

  • hsa-miR-21 may negatively regulate LRIG1 expression in LUAD, influencing cancer onset and progression.
  • The hsa-miR-21/LRIG1 axis is a potential prognostic biomarker and therapeutic target in LUAD.
  • This interaction impacts the tumor microenvironment, specifically immune cell infiltration.