Chemical constituents from symplocos sumuntia and their anti-inflammatory effect

Symplocos sumuntia Buch-Ham ex D Don is traditionally used to relieve inflammation in Vietnam. This study described a chemical investigation that led to isolation and identification of one new and nine known compounds. Their structures were determined by using spectroscopic techniques. Compounds 1 - 10 possessed inhibitory activities on NO production induced by LPS activated macrophages. Among them, compounds 2 and 3 demonstrated strong ability to reduce NO production with IC₅₀ values of 3.91 ± 0.05 and 3.96 ± 0.24 µM, respectively. Some of the other compounds also reduced NO production in LPS stimulated RAW264.7 cells. In-silico study supported hints to the bioactivity of compounds 2 and 3 by analysing the interactions of those when they were docked with protein to create complexes targeting anti-inflammation. Our research revealed the chemical components of this plant and its active ingredients that target inflammation, highlighting its therapeutic potential to improve its value for traditional uses.