Related Experiment Videos
[Interference of polyester inflammation on the development of Yoshida ascites hepatoma in the rat]
Abstract:
Minced polyester threads introduced into the peritoneal cavity cause a chronic inflammation with evidence of macrophage and lymphocyte stimulation. In this paper an interference between this kind of inflammation and the growth of Yoshida ascites hepatoma has been shown, which has been found to dependent on the time interval elapsed between the introduction of polyester (Mersilene) minces and injection of the hepatoma cells. Rats were treated intraperitoneally with Mersilene and then divided in to three groups: the first was injected intraperitoneally with hepatoma cells immediately (TM 0), the second after 7 (TM 7) and the third after 14 days (TM 14); rats untreated with polyester and implanted with the same number of hepatoma cells served as controls (NT). While in groups NT and TM 0 a rapid growth of hepatoma cells occurred, together with the accumulation of a considerable volume of ascitic fluid, no tumor growth neither ascite production occurred in groups TM 7 and TM 14; in these animals where several days were allowed to elapse after polyester introduction, the hepatoma cells which had been injected rapidly disappeared and were no more found 48 h after the intraperitoneal injection. It is suggested that the inhibition of the neoplastic growth may be dependent on the activation of macrophages (and possibly NK cells) which accompanies the development of the chronic polyester inflammation.
Insights
Chronic inflammation induced by polyester (Mersilene) threads inhibits Yoshida ascites hepatoma growth in rats. This anti-tumor effect, observed after a delay, is linked to macrophage activation.
Area of Science:
- Oncology
- Immunology
- Biomaterials Science
Background:
- Peritoneal implantation of minced polyester threads induces chronic inflammation.
- Inflammation involves macrophage and lymphocyte stimulation.
- The interaction between chronic inflammation and tumor growth is not fully understood.
Purpose of the Study:
- To investigate the interference between polyester-induced chronic inflammation and Yoshida ascites hepatoma growth.
- To determine the role of the time interval between polyester implantation and tumor cell injection.
Main Methods:
- Rats were intraperitoneally treated with Mersilene (polyester) minces.
- Hepatoma cells were injected at different time points (0, 7, 14 days) post-Mersilene treatment.
- Control groups received hepatoma cells without prior Mersilene treatment.
- Tumor growth and ascitic fluid accumulation were monitored.
Main Results:
- Rapid hepatoma cell growth and ascites occurred in control and immediate treatment (TM 0) groups.
- No tumor growth or ascites was observed in groups treated 7 (TM 7) and 14 (TM 14) days prior to tumor cell injection.
- Hepatoma cells injected into rats with established polyester inflammation disappeared within 48 hours.
Conclusions:
- A time-dependent inhibition of Yoshida ascites hepatoma growth occurs following polyester implantation.
- This anti-neoplastic effect is likely mediated by macrophage activation (and potentially NK cells) associated with chronic polyester-induced inflammation.