The Effects of Chemically Modified Biochar on Biomethane Production from Glucose and Sugar Beet Pulp

The research aimed to study the effects of straw-derived biochar and two types of chemically modified biochar on biomethane production from glucose as a model substrate and sugar beet pulp as a real substrate. The biochar chemical modification with H₃PO₄ acid and KOH base resulted in a change in biochar surface area properties and its functional group's abundance and a decrease in biochar mass yield production. The anaerobic digestion process was performed in batch reactors kept at 37 °C for 20 days. The substrate-to-inoculum ratio by volatile solids was 0.5, while the mass of added biochar corresponded to 16 g·L^-1. The results showed that neither the addition of biochar nor the chemically modified biochar had any positive effects on biomethane production or its kinetics in the case of both substrates. The highest methane production was found in reactors without biochar added, respectively, 385 and 324 mL·g_VS^-1 for glucose and sugar beet pulp. It is hypothesized that the anaerobic digestion process was performed under optimal conditions, and therefore, biochar could not enhance methane production. Additionally, biochar may have adsorbed some volatile fatty acids, making them less available to anaerobic microorganisms.