Hierarchical MXene/Enzyme@Covalent Organic Framework Composite by Template Method for Photothermal-Enhanced Lipase...

Area of Science:

• Biocatalysis
• Materials Science
• Chemical Engineering

Background:

• Enzyme immobilization is crucial for industrial biomolecule applications but faces challenges in activity, stability, and scalability.
• Existing methods struggle with enzyme leaching, conformational changes, and efficient recovery.

Purpose of the Study:

• To develop advanced enzyme immobilization techniques for efficient, stable, and tunable catalysis.
• To create a novel enzyme membrane reactor overcoming limitations of previous immobilization systems.

Main Methods:

• Synthesized enzyme@cyclodextrin covalent organic framework (COF) films via a template method.
• Constructed a 3D hydrogen bond-enhanced MXene/enzyme membrane reactor using the COF films.
• Utilized MXene for interfacial photothermal properties and enhanced mass transfer.

Main Results:

• The new strategy provides a spacious microenvironment, simplifying the process and improving biocompatibility.
• Obtained biocatalysts exhibit excellent reusability, high activity, and stability.
• The enzyme membrane reactor, a 'smart capsule', effectively addresses previous enzyme immobilization challenges.

Conclusions:

• Developed a novel, green, efficient, stable, and sustainable enzyme catalytic platform.
• The enzyme@cyclodextrin-COF and MXene composite membrane reactor offers a versatile solution for industrial biocatalysis.
• This approach provides a new paradigm for enzyme immobilization and application.