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Updated: May 10, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies
Mercedes Paz1,2, Gonzalo Moratorio1,2,3
1Laboratory of Experimental Virus Evolution, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Abstract:
Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensive mutagenesis and detailed functional characterization of viral proteins. These approaches have significantly deepened our understanding of the molecular determinants driving viral evolution and adaptation. Furthermore, we discuss how these advances provide transformative insights for future vaccine development and therapeutic strategies.
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