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Related Concept Videos

CRISPR01:59

CRISPR

48.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: May 10, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies.

Mercedes Paz1,2, Gonzalo Moratorio1,2,3

  • 1Laboratory of Experimental Virus Evolution, Institut Pasteur de Montevideo, Montevideo, Uruguay.

Msphere
|April 24, 2025
PubMed
Summary

Synthetic biology and sequencing advance viral genome manipulation. Deep mutational scanning and CRISPR genome editing enhance understanding of viral evolution, aiding future vaccine and therapeutic strategies.

Keywords:
CRISPR-engineered virusesdeep mutational scanningvirus evolution

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Area of Science:

  • Virology
  • Synthetic Biology
  • Molecular Biology

Background:

  • Synthetic biology and sequencing technologies have greatly improved the manipulation of viral genomes.
  • Understanding viral evolution and adaptation is crucial for public health.

Purpose of the Study:

  • To review advanced methodologies for viral genome manipulation.
  • To highlight the functional characterization of viral proteins.
  • To discuss implications for vaccine development and therapeutics.

Main Methods:

  • Deep mutational scanning for comprehensive mutagenesis.
  • CRISPR-based genome editing for precise genetic modification.
  • Functional characterization of viral proteins.

Main Results:

  • These methods allow for detailed analysis of viral protein function.
  • A deeper understanding of the molecular basis of viral evolution and adaptation has been achieved.
  • Insights into viral pathogenesis and host interactions.

Conclusions:

  • Advanced genomic tools offer unprecedented precision in studying viral systems.
  • These techniques are pivotal for developing novel antiviral therapies and vaccines.
  • Future research directions in viral genomics and engineering.