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Related Experiment Video

Updated: May 10, 2025

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
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High-Throughput Screening of Polyfluoroalkyl Substances Using Solid-Phase Microextraction Coupled to Microfluidic

Wei Zhou1, Malvika Dutt2, Qizhen Lan1

  • 1Department of Chemistry, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

Environmental Science & Technology
|April 24, 2025
PubMed
Summary

A new high-throughput system enables rapid and sensitive detection of 18 per- and polyfluoroalkyl substances (PFAS) in various matrices. This method uses solid-phase microextraction (SPME) coupled with mass spectrometry (MS) for efficient environmental and public health monitoring.

Keywords:
PFAShigh-throughputmicrofluidic open interfacerapid screeningsolid-phase microextractionwater analysis

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Toxicology

Background:

  • Per- and polyfluoroalkyl substances (PFAS) pose significant environmental and public health risks.
  • Current large-scale monitoring methods for PFAS are often inefficient and time-consuming.
  • Sensitive and rapid detection techniques are crucial for effective PFAS remediation and regulation.

Purpose of the Study:

  • To develop a high-throughput, automated sample preparation and analysis system for PFAS.
  • To achieve sensitive detection of a wide range of PFAS in diverse sample matrices.
  • To optimize the method for speed, efficiency, and broad applicability in environmental monitoring.

Main Methods:

  • Development of a 48-sample high-throughput system utilizing solid-phase microextraction (SPME) blades.
  • Direct coupling of SPME to mass spectrometry (MS) via an automated microfluidic open interface (MOI).
  • Elimination of chromatographic separation, enabling rapid analysis (2.8 min/sample).

Main Results:

  • Sensitive detection of 18 PFAS in drinking water with limits of detection (LODs) from 1 to 10 pg/mL.
  • Successful PFAS screening in complex matrices including blood, beer, and beef.
  • Identified and addressed issues with low recovery and reproducibility for nonpolar PFAS in water analysis.

Conclusions:

  • The SPME-MOI-MS system offers a highly efficient and sensitive approach for large-scale PFAS monitoring.
  • The method's versatility allows for analysis across various challenging sample types.
  • Optimizations involving glass containers and acetonitrile improve the reliability of nonpolar PFAS detection.