Recyclable cation exchange resin-driven fermentation of waste activated sludge in sequential batch-parallel pattern:...

Anaerobic acidogenic fermentation has been posited as a preferable technology for waste activated sludge management, whereas the inefficient hydrolysis, inadequate metabolism and disordered microbiota still existed as three fermentability limitations. The existing solutions addressed single limitation while consuming substantial chemicals/energy, thereby restraining technological dissemination. Innovatively, the recyclable cation exchange resin (CER) is a promising approach for synchronously overcoming these fermentability limitations and reducing chemicals/energy costs from sludge-native metal removal perspective; however, it has been rarely reported. This study pioneered a recyclable CER-driven sludge fermentation in continuous CER and regenerant reuse scene, taking comprehensive insights into long-term performance and multiple mechanisms. The CER induced speciation conversion and stepwise removal of structural metals from sludge, especially organic-binding and residual Ca&Mg, which played triple driving contributions: (1) breaking metal-bridging sites and hydrogen bonds disentangled protein molecules for raising electronegative repulsion and flocculation energy barrier, causing synergic extracellular and intracellular hydrolysis (up to 30.05 %); (2) liberating endogenous redox mediators from metal-complexations for assisting electron shuttle and extracellular respiration, which metabolic electron transfer activity by 1.58 times; (3) triggering "bacteria screening" through sensitive methanogen inhibition and tolerant acidogens growth towards maximum acidogenic eco-functions. Such fermentability breakthroughs greatly promoted short-chain fatty acids (superior carbon sources) accumulation by average 2.52 folds while declining sludge solid by 55.87 %. The NaCl regeneration thoroughly restored CER active sites and eluted pollutant blockages, with negligible capability loss ≤ 3.53 % in 18-cycle operations, which stabilized acidogenic performances during 72-day fermentation (RSD ≤ 7.88 %). The fermentative products presented as high-quality carbon sources with abundant carbon and absent nitrogen, owing to CER-mediated NH₄⁺ exchange. Innovative batch-parallel operation was established in engineering strategy, offering 409.49 CNY/ton SS income. The findings provided mechanism framework linking sludge fermentability with native metal functions.