Dual-omics analysis of transcriptome and translatome unveils lycopene-induced triglyceride accumulation mechanisms in...

Intramuscular fat is a critical determinant of meat taste and flavor. Therefore, regulating intramuscular fat content to enhance meat quality is considered a viable strategy. Lycopene, a natural antioxidant, has been shown to improve the oxidative stability and color of meat, both of which are crucial for maintaining meat product quality. Previous studies have suggested that lycopene may contribute to improved meat quality by influencing intramuscular fat deposition. However, the specific mechanisms underlying lycopene's effects on intramuscular fat deposition remain unclear and require further investigation. This study aimed to elucidate the molecular mechanisms through which lycopene regulates intramuscular fat deposition in muscle cells and to identify differentially expressed genes involved in this process. Using integrated transcriptomic and translatomic dual-omics analyses of C2C12 myoblasts treated with or without lycopene, we identified 55 functional genes implicated in transcriptional and translational regulation. Our findings suggest that lycopene may modulate lipid metabolism in C2C12 myoblasts by regulating the expression of Ankrd1. This study not only provides valuable insights into the coordinated regulation of gene expression at both the transcriptional and translational levels but also establishes a theoretical foundation for the beneficial role of lycopene in improving meat quality.