Dual-channel fluorescent probe for simultaneously visualizing ONOO- and viscosity in epilepsy, non-alcoholic fatty liver and tumoral ferroptosis models
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View abstract on PubMed
Summary
This summary is machine-generated.A novel near-infrared fluorescent probe, QX-DP, enables simultaneous detection of intracellular peroxynitrite anion (ONOO-) and viscosity. This dual-biomarker approach aids in diagnosing diseases like epilepsy, liver injury, and tumor ferroptosis.
Area Of Science
- Biomedical Engineering
- Chemical Biology
- Molecular Imaging
Background
- Intracellular peroxynitrite anion (ONOO-) and viscosity are crucial for cellular functions like redox homeostasis and signal transduction.
- Dysregulation of ONOO- and viscosity is linked to various pathological conditions, including epilepsy and ferroptosis.
- Current limitations in multifunctional fluorescent sensors hinder the simultaneous detection of ONOO- and viscosity in disease models.
Purpose Of The Study
- To develop a novel near-infrared (NIR) fluorescent probe for the concurrent detection of ONOO- and viscosity.
- To validate the probe's efficacy in visualizing and diagnosing diseases characterized by altered ONOO- and viscosity levels.
Main Methods
- Rational design and synthesis of a new NIR fluorescent probe, QX-DP.
- Characterization of QX-DP's sensitivity and selectivity towards ONOO- and viscosity.
- Application of QX-DP for dual-channel fluorescence imaging in disease models, including epilepsy, non-alcoholic liver injury (NAFL), and tumoral ferroptosis.
Main Results
- QX-DP demonstrated high sensitivity to viscosity (668 nm) and ONOO- (752 nm) with significant "turn-on" fluorescence signals.
- The probe successfully revealed elevated ONOO- and viscosity levels in the brain tissue of epileptic mice.
- QX-DP enabled visualization of NAFL disease models and concurrent imaging of tumor ferroptosis in cancer cells, zebrafish, and tumor mice models.
Conclusions
- The developed QX-DP probe offers a powerful tool for simultaneous monitoring of ONOO- and viscosity.
- Dual-biomarker detection provides a more sensitive and reliable strategy for diagnosing and imaging diseases related to oxidative stress and viscosity.
- This approach holds significant potential for image-assisted surgery and understanding complex pathological processes.
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