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Updated: May 10, 2025

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
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Enhancing Doxorubicin Sensitivity in Osteosarcoma Cancer Cells: Unveiling the Role of Resveratrol-Induced Oxidative

Mohammad Reza Bazavar1, Hamed Helali1, Linda Mohammadzadeh Boukani2

  • 1Department of Orthopedic Surgery, School of Medicine and Shohada Educational Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.

Drug Research
|April 24, 2025
PubMed

Insights

Resveratrol enhances doxorubicin-induced apoptosis in Saos-2 cells by increasing DNA damage markers. This suggests Resveratrol may aid osteosarcoma treatment by boosting chemotherapy effects.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Doxorubicin (DOX) is a chemotherapy agent used to treat osteosarcoma.
  • DOX-induced DNA damage can lead to apoptosis in cancer cells.
  • Resveratrol (Resv) is a natural polyphenol with potential anti-cancer properties.

Purpose of the Study:

  • To investigate the effect of Resveratrol on doxorubicin-induced apoptosis in Saos-2 osteosarcoma cells.
  • To determine if Resveratrol influences DNA damage pathways activated by doxorubicin.

Main Methods:

  • Saos-2 cells were treated with DOX and Resveratrol.
  • Cell viability was assessed using MTT assay.
  • DNA damage markers (H2AX, ATR, ATM, Rad51, p53) were quantified by qRT-PCR and Western blotting.
  • Apoptosis was analyzed via flow cytometry.

Main Results:

  • Doxorubicin significantly suppressed Saos-2 cell proliferation in a dose-dependent manner.
  • Resveratrol enhanced DOX-induced apoptosis in Saos-2 cells (P<0.05).
  • Expression of DNA damage markers H2AX, ATR, ATM, Rad51, and p53 increased, potentially mediating apoptosis.

Conclusions:

  • Resveratrol potentiates doxorubicin-induced apoptosis in Saos-2 cells.
  • The findings suggest Resveratrol may enhance the efficacy of doxorubicin in osteosarcoma treatment.
  • Resveratrol's role in modulating DNA damage response pathways warrants further investigation for therapeutic applications.

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