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Updated: May 14, 2025

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC
Published on: May 10, 2016
Network toxicological analysis of sodium dehydroacetate in food safety
Jing Jin1, Yan Xue2, Liang Tian1
1Center for Rehabilitation Medicine, Department of Rehabilitation, Neurotumor Rehabilitation Ward, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Abstract:
Sodium dehydroacetate (Na-DHA), a synthetic preservative under tightened regulations, was evaluated for multi-organ toxicity using network toxicology. ADMETlab3.0 predicted genotoxicity, hepatotoxicity, and carcinogenicity risks. Target mining identified 13 cancer-related, 11 liver injury-related, and 8 genotoxicity-related core genes, with shared hubs (ALOX5, PTGS2, SMAD3, TNF) across pathologies. Functional analyses revealed inflammation, oxidative stress, and immune dysregulation as central mechanisms. KEGG pathway analysis linked cancer/liver injury to AGE-RAGE signaling (TNF, NOX4) and genotoxicity to efferocytosis impairment (PTGS2, ALOX5), suggesting DNA repair disruption. The integrated network demonstrated Na-DHA's pleiotropic effects through convergent pathways, transcending organ-specific toxicity. This systemic profile challenges conventional single-endpoint assessments, advocating comprehensive multi-organ risk evaluation.
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