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Updated: May 10, 2025
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Identification and genome sequence analysis of a novel alphabaculovirus isolated from sweet potato leaf worm, Aedia
Jian Yang1, Zhigao Zhan1, Wendong Kuang1
1Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, Jiangxi, China.
Abstract:
Sweet potato leaf worm (Aedia leucomelas; Lepidoptera: Noctuidae) adversely affects sweet potato quality and yield. We collected infected A. leucomelas larvae with typical symptoms of baculovirus infection in Fuzhou, China in 2023 and then isolated a baculovirus, which we named Aedia leucomelas nucleopolyhedrovirus (isolate Fuzhou;AeleNPV-FZ). Helicoverpa armigera and Spodoptera exigua larvae were also susceptible to AeleNPV-FZ in laboratory assays, suggesting that AeleNPV-FZ may be useful for controlling multiple noctuid pests under field conditions. The AeleNPV-FZ genome was sequenced and de novo assembled. The genome was 135,409 bp in length with 146 open reading frames (ORFs) and 12 homologous regions (hrs) annotated for the sequence. Phylogenetic relationships inferred from concatenated core gene amino acid sequence alignment and pairwise Kimura two-parameter distances indicated that AeleNPV-FZ may be a novel member of genus Alphabaculovirus.
