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Dual-channel, real-time, long-term stable electrochemical immunosensor based on Au, Cu-vertical graphene for detection of carcinoembryonic antigen in tumor cells

  • 0Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin Key Laboratory of Life and Health Detection, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, PR China.
Analytica Chimica Acta +

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April 24, 2025

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April 24, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A novel dual-channel electrochemical immunosensor using an Au-AuCu-vertical graphene platform enables sensitive and rapid detection of carcinoembryonic antigen (CEA). This advancement aids in early cancer diagnosis and monitoring treatment efficacy.

Area Of Science

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background

  • Accurate determination of carcinoembryonic antigen (CEA) is crucial for cancer diagnosis, drug development, and prognosis.
  • Existing methods for CEA detection face challenges in sensitivity, speed, and on-site applicability.

Purpose Of The Study

  • To design and develop a sensitive dual-channel electrochemical immunosensor for the rapid determination of CEA levels.
  • To improve the performance of CEA detection using a novel Au-AuCu-vertical graphene (VG) sensing platform.

Main Methods

  • Fabrication of a sensing platform using Au-AuCu-vertical graphene (VG) with electrodeposited gold nanoparticles.
  • Immobilization of CEA antibodies onto the gold nanoparticles for specific antigen binding.
  • Development of a dual-channel electrochemical immunoassay with custom software for real-time analysis.

Main Results

  • The immunosensor demonstrated a wide linear range (0.001 to 30000 pg mL⁻¹) and a low limit of detection (0.28 fg mL⁻¹).
  • The developed sensor exhibited excellent selectivity, reproducibility, and long-term stability.
  • High-precision, real-time on-site analysis of CEA concentrations was achieved.

Conclusions

  • The AuCu-VG electrode effectively binds antibodies and gold nanoparticles, providing a robust platform for immunosensing.
  • A novel dual-channel electrochemical immunoassay strategy enables sensitive and reliable detection of CEA in real-time.
  • This technology holds promise for improved cancer diagnostics and patient monitoring.

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