Related Experiment Video
Updated: Apr 26, 2026

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
Published on: November 16, 2012
Engineering a multi-epitope chimera derived from Acinetobacter baumannii CAM87009.1 and FilF fimbriae proteins:
Pamela Scaraffuni Caballero1, Isabel Ladeira Pereira1, Stella Buchhorn de Freitas1
1Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, Pelotas, RS, CEP 96010-900, Brazil.
Abstract:
This study aimed to develop a multi-epitope recombinant chimera (rChimera) based on two outer membrane proteins of Acinetobacter baumannii, targeting passive or active immunization strategies against biofilm-forming strains of this multidrug-resistant bacterium. Using bioinformatics and reverse vaccinology, we identified CAM87009.1 and FilF as two conserved proteins involved in biofilm formation and host cell adherence. An in silico analysis was performed to design the rChimera, selecting 36 promising B- and T-cell epitopes, including those recognized by MHC class I and II. These epitopes were linked using a glycine linker (GGGG) and a rigid linker (EAAKEAAAKA). The chimera was chemically synthesized, successfully expressed in Escherichia coli BL21 Star, and recognized by antibodies from naturally infected patients. Polyclonal antibodies (pAbs) produced in a murine model elicited a significant IgG response from the 14th day after the first immunization, with IgG1 and IgG2 (a and b) being the predominant isotypes generated. Anti-rChimera pAbs effectively inhibited biofilm formation in multidrug-resistant isolates and the ATCC® 19,606 strain of A. baumannii. The recombinant chimera shows promise for passive or active immunization against biofilm-forming strains of A. baumannii.
Related Concept Videos
Cytoskeletal Proteins in Bacteria
Mechanism of Filopodia Formation
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Fimbriae, Pili, and Axial Filaments
Biofilms

