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Related Experiment Video

Updated: May 10, 2025

Collection and Identification of Pollen from Honey Bee Colonies
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Pollen-Microbe Interactions in Nectar Weakly Influence Bee Foraging Behavior.

Success C Ekemezie1, Charlotte C Davis1, Marco V Russo1

  • 1Department of Biology, Missouri State University, Springfield, MO 65897, USA.

Integrative and Comparative Biology
|April 25, 2025
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Summary
This summary is machine-generated.

Flower microbes and pollen interactions in nectar did not alter bee flower preference or constancy. However, bees rejected nectar contaminated with microbes and pollen, suggesting minor impacts on pollinator foraging behavior.

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Area of Science:

  • Ecology
  • Microbiology
  • Behavioral Ecology

Background:

  • Floral microbes, including bacteria and yeast, commonly inhabit nectar.
  • Nectar microbes can interact with pollen, potentially altering nutrient availability for pollinators.

Purpose of the Study:

  • To investigate the impact of nectar microbes (yeast Metschnikowia reukaufii, bacteria Acinetobacter nectaris) on pollen germination and bursting.
  • To determine how these pollen-microbe interactions affect bumble bee (Bombus impatiens) foraging behavior.

Main Methods:

  • Artificial nectar experiments were used to assess pollen germination and bursting in the presence of microbes.
  • Bumble bee foraging behavior was observed in response to nectar treatments with varying levels of pollen and microbial contamination.

Main Results:

  • Both yeast and bacteria reduced intact pollen, with bacteria causing the most germination and bursting.
  • Bees did not show altered flower preference or constancy towards microbe-contaminated nectar.
  • Bees significantly rejected flowers with nectar contaminated by pollen and yeast, or pollen, yeast, and bacteria.

Conclusions:

  • Pollen-microbe interactions in nectar may have limited direct effects on pollinator preference and constancy.
  • Floral nectar contamination by microbes and pollen can lead to nectar rejection by pollinators.
  • These findings contribute to understanding plant-pollinator ecology and the role of microbial communities.