Optimized Microfluidic Biosensor for Sensitive C-Reactive Protein Detection.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Point-of-Care Diagnostics

Background:

• Traditional lateral flow immunoassays (LFIA) face limitations in sensitivity and detection range for C-reactive protein (CRP).
• Current methods often require multiple steps and buffer solutions, increasing complexity and potential for error.
• Optimizing fluid dynamics is crucial for improving assay performance in point-of-care testing (POCT).

Purpose of the Study:

• To enhance the sensitivity and expand the detection range of CRP using microfluidic-integrated LFIA.
• To develop a simplified, one-step detection system for CRP suitable for POCT.
• To improve the efficiency and reduce resource requirements for CRP diagnostics.

Main Methods:

• Integration of LFIA into microfluidic chips for precise fluid control.
• Utilized gold nanoparticle and fluorescent labels for CRP detection across different concentration ranges.
• Minimized nitrocellulose pad area and eliminated buffer solution steps.

Main Results:

• Microfluidic control enhanced CRP detection sensitivity and accuracy.
• Gold nanoparticle method achieved high sensitivity (1-10 μg/mL) for chronic disease monitoring.
• Fluorescent labels extended the CRP detection range to 1-70 μg/mL, covering infection levels.
• Reduced non-specific binding and eliminated the need for pad blocking.

Conclusions:

• Microfluidic integration significantly improves LFIA performance for CRP detection.
• The developed system offers enhanced operational efficiency, reduced resource use, and greater control over fluid dynamics.
• This approach is suitable for resource-limited settings, paving the way for accessible and cost-effective POCT.