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An Orthotopic Model of Murine Bladder Cancer
Published on: February 6, 2011
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Pulsed Electromagnetic Field Therapy Alters the Genomic Profile of Bladder Cancer Cell Line HT-1197
Maxwell Sandberg1, Wyatt Whitman1, Randall Bissette1,2
1Atrium Health Wake Forest Baptist Medical Center, Winston Salem, NC 27101, USA.
Journal of Personalized Medicine
|April 25, 2025
Summary
Pulsed electromagnetic field (PEMF) therapy slowed bladder cancer cell growth and altered gene expression in HT-1197 cells. This suggests PEMF therapy may be a promising new treatment for bladder cancer.
Area of Science:
- Oncology
- Biophysics
- Cell Biology
Background:
- Pulsed electromagnetic field (PEMF) therapy utilizes magnetic waveform energy for targeted treatment.
- PEMF therapy has shown potential in treating various cancers.
- Current bladder cancer treatments are invasive; PEMF offers a potential alternative.
Purpose of the Study:
- To investigate the mechanistic effects of PEMF therapy on bladder cancer cells.
- To examine the impact of PEMF on cell proliferation and gene expression in vitro.
Main Methods:
- Bladder cancer cell line HT-1197 was cultured and exposed to PEMF daily for five days.
- Cell proliferation was assessed using Incucyte® data.
- Gene expression analysis was performed on days 1 and 5 post-treatment.
Main Results:
- PEMF therapy significantly reduced proliferation rates in HT-1197 cells (p < 0.05).
- Principal component analysis indicated distinct gene expression profiles between treated and untreated cells.
- Numerous cancer-related genes and pathways showed altered expression following PEMF treatment.
Conclusions:
- PEMF therapy demonstrably slowed proliferation and altered gene expression in HT-1197 bladder cancer cells.
- Several cancer-relevant pathways were differentially regulated by PEMF treatment.
- PEMF therapy warrants further investigation as a potential bladder cancer treatment, despite limitations.
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