Related Experiment Video

Updated: May 10, 2025

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
08:02

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Published on: January 6, 2012

28.5K

OTUD1 inhibits endometriosis fibrosis by deubiquitinating MADH7

Xiangyu Chang1, Yanqin Zhang1, Mengqi Deng1

  • 1Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.

Molecular Human Reproduction
|April 25, 2025
PubMed

Insights

Ovarian tumor domain-containing protein 1 (OTUD1) inhibits endometriosis fibrosis by deubiquitinating MADH7. This finding reveals OTUD1 as a potential therapeutic target for reducing endometriosis-related pain and infertility.

Area of Science:

  • Reproductive biology
  • Molecular oncology
  • Cellular biology

Background:

  • Fibrosis is a key factor in endometriosis-associated pain and infertility.
  • MADH7, a TGF-β pathway inhibitor, influences fibrosis progression.
  • The role of Ovarian tumor domain-containing protein 1 (OTUD1) in endometriosis is not well understood.

Purpose of the Study:

  • To investigate the role of OTUD1 in endometriosis.
  • To determine OTUD1's impact on endometriosis lesion growth in vitro and in vivo.

Main Methods:

  • Utilized C57BL/6N female mice and human primary endometrial stromal cells (HEMCs).
  • Assessed OTUD1's effect on fibrosis-related protein expression in HEMCs.
  • Investigated the mechanism involving deubiquitination and MADH7-mediated transcriptional reprogramming.

Main Results:

  • OTUD1 inhibited fibrosis-related protein expression in HEMCs.
  • OTUD1 deubiquitination of MADH7 reprogrammed transcription, halting lesion growth in vivo.
  • Reduced abdominal inflammation was observed in vivo.

Conclusions:

  • OTUD1 plays a critical role in regulating fibrosis in endometriosis.
  • OTUD1's mechanism involves MADH7-mediated transcriptional reprogramming.
  • OTUD1 demonstrates potential as a therapeutic agent for endometriosis.
Keywords:
MADH7OTUD1deubiquitinationendometriosisfibrosismice model

More Related Videos

A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice07:12

A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice

Published on: November 24, 2020

5.0K
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice07:44

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Published on: February 17, 2023

1.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
2.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K