Related Experiment Video
Updated: Jul 30, 2026

Highly-Multiplexed Tissue Imaging with Raman Dyes
Published on: April 21, 2022
TissueViewer: a web-based multiplexed image viewer
David Gerardus Pieter van IJzendoorn1, Magdalena Matusiak1, Rob West1
1Department of Pathology, Stanford University, Stanford, CA 94305, United States.
Motivation:
Datasets generated by spatial biology techniques such as multiplex immunofluorescence staining or spatial transcriptomics profiling of histologic sections carry a tremendous wealth of information. Several commercial platforms exist that can simultaneously acquire 1-1000 distinct marker signals (e.g. MIBI, CODEX, Orion, Nanostring CosMX SMI, Vizgen). However, due to the large size of these datasets, their viewing and sharing are slow, laborious, and require extensive computational resources.
Results:
To overcome these challenges, we developed TissueViewer, an easy to setup and use web-based viewer designed to deliver high-resolution images over the internet with low bandwidth requirements and at high speed.
Availability And Implementation:
TissueViewer is available on GitHub (https://github.com/davidvi/tissueviewer) and can be used on the TissueViewer.org platform, where readers can upload their own data with a limit of 50 GB to share with colleagues.

