Related Experiment Video
Updated: Jul 13, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
Published on: February 13, 2016
Deciphering membrane biofouling induced by micro-/nano-plastics in nanofiltration: Metagenomic insights and
Xinhui Liu1, Yu Yang1, Nigel J D Graham2
1School of Environment, Beijing Normal University, Beijing, 100875, China.
Abstract:
Nanofiltration (NF) is an effective process for micro-/nano-plastics (MNPs) interception, but the impact of accumulated MNPs on the microbial community structure and metabolic pathways of biofilms on NF membranes remains unclear. This provides uncertainty with respect to membrane biofouling behavior and the risks to efficient NF operations. In this study, the size-dependent (20 nm-25 microm) and concentration-dependent (0.1-50 mg·L-1) effects of MNPs on the biofouling of a NF membrane treating secondary wastewater effluent were studied. Three MNPs-tolerant, hypermetabolic and polystyrene-degradable genera (i.e., Acinetobacter, Novosphingobium and Asticcacaulis) were detected in biofilms as dominant taxonomic compositions. MNPs led to an increase of 19.3 %-76.7 % in biomass contents and a more rapid decrease in permeate flux, with 0.1 mg·L-1 of 80 nm NPs causing the most severe membrane biofouling. Metagenomic analysis revealed that MNPs upregulated enzymes involved in exopolysaccharide (ExoA/L/M/P/Q/X/Y/Z) and tyrosine (COMT, FeaB and AOC3) biosynthesis and quorum sensing (PhzF and CiaH/R), and suppressed cell motility pathways including flagellar assembly and bacterial chemotaxis. Novel types of perforated column spacer (PCS) enhanced the hydrodynamics of the membrane feed with a lower pressure drop and higher fluid velocity, introduced micro-jets and greater mass transfer inside feed channels, thus eliminating the deposition of MNPs and mitigating membrane biofouling. Overall, a greater understanding of the interaction mechanisms between MNPs and membrane biofouling in secondary effluent filtration will help develop more effective MNPs management strategies and achieve more sustainable NF operations.
More Related Videos
05:31Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris
Published on: July 28, 2018
08:21Forming Micro-and Nano-Plastics from Agricultural Plastic Films for Employment in Fundamental Research Studies
Published on: July 27, 2022
Related Concept Videos
Biofilms
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Microbial Wastewater Treatment
Microbial Corrosion
Microbial Bioremediation of Plastics