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Updated: May 10, 2025
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
A fluorometric and naked eye readable neuraminidase probe for influenza virus detection
Hao Chang1, Xin Zhao2, Zhenyou Jiang3
1Department of Laboratory Medicine, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, China; Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China.
Abstract:
Rapid diagnostic methods are crucial for effective influenza therapy and the prevention of flu pandemics. Neuraminidase (NA), a key protein on the surface of influenza virions, plays a vital role in the replication of the influenza virus (IV). Herein, we present SANP, a multimode NA-activatable fluorescent probe designed for rapid influenza detection and live-cell imaging. This probe demonstrated a high sensitivity with a limit of detection (LOD) of 15.4 mU/mL for NA and 2-2 hemagglutination units (0.25 HAU) for influenza virus samples. SANP also exhibited a LOD of 20 HAU (1 HAU) when detected influenza virus samples by the naked eye without the aid of equipment. Moreover, SANP facilitated real-time visualization of NA activity in influenza-infected cells through fluorescence imaging.
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