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Updated: May 16, 2025
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
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Long non-coding RNA and mRNA expression profiling of porcine satellite cells using strand-specific RNA-seq
Cencen Li1, Xiaofang Cheng1, Tianyu Li1
1Department of Biotechnology, college of Life Sciences, Xinyang normal University, Xinyang, China.
Scientific Data
|April 25, 2025
Summary
This study identifies key long non-coding RNAs (lncRNAs) regulating porcine satellite cell (PSC) proliferation and differentiation. The findings advance understanding of molecular mechanisms in muscle development.
Area of Science:
- Molecular Biology
- Genomics
- Developmental Biology
Background:
- Skeletal muscle satellite cells are crucial for muscle growth and repair.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cellular differentiation.
- Limited research exists on lncRNAs governing porcine satellite cell (PSC) functions.
Purpose of the Study:
- To identify and characterize lncRNAs involved in PSC proliferation and differentiation.
- To provide a comprehensive expression profile of lncRNAs and mRNAs during PSC development.
- To elucidate the molecular basis of porcine muscle development.
Main Methods:
- Strand-specific RNA sequencing (RNA-seq) was employed.
- PSC samples were collected at distinct proliferation and differentiation time points (P24h, P48h, D18h, D28h).
- Bioinformatic analysis was performed on sequencing data to identify lncRNAs and mRNAs.
Main Results:
- Over 61 million clean reads were generated per sample on average.
- 1950 novel and 9367 annotated lncRNAs were identified.
- 57252 mRNA transcripts were detected, offering a rich dataset for further study.
Conclusions:
- This research provides valuable insights into the function of lncRNAs in PSC differentiation.
- The identified lncRNAs are potential regulators of porcine muscle development.
- This study enhances the understanding of the molecular mechanisms governing muscle biology.
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