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Single-cell epigenetics and multiomics analysis in kidney research.
Seishi Aihara1, Yoshiharu Muto2
1Division of Nephrology, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5901 Forest Park Rd., Dallas, TX, 75390, USA.
Clinical and Experimental Nephrology
|April 25, 2025
Summary
Single-cell sequencing and multiomics analysis reveal kidney cell diversity and gene regulation. These advanced techniques offer new biomarkers and therapeutic targets for kidney diseases.
Area of Science:
- Nephrology
- Genomics
- Epigenetics
Background:
- Single-cell sequencing advances understanding of kidney cellular heterogeneity.
- Transcriptomic data alone limits deciphering complex gene regulatory mechanisms.
- Emerging single-cell multiomics approaches integrate epigenome and transcriptome data.
Purpose of the Study:
- To review single-cell sequencing analysis in nephrology.
- To highlight emerging epigenomic and multiomics approaches.
- To discuss their application in kidney research.
Main Methods:
- Review of single-cell transcriptomic analysis.
- Focus on single-cell epigenomic and multiomics approaches.
- Integration of epigenome and transcriptome data for regulatory insights.
Main Results:
- Single-cell multiomics enables dissection of cell-specific regulatory mechanisms.
- Generation of single-cell multimodal atlases in nephrology research.
- Identification of novel biomarkers and therapeutic targets.
Conclusions:
- Single-cell multiomics provides deeper insights into kidney cellular diversity and epigenetic regulation.
- This paradigm shift accelerates biomarker and therapeutic target discovery.
- Knowledge of these approaches is crucial for researchers and nephrologists.
