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Related Experiment Videos

Developmentally regulated cell surface structures on mouse and human embryonal carcinoma cell lines.

P Avner, D Arnaud, C Blaineau

    Cell Differentiation
    |July 1, 1985
    PubMed
    Summary

    Three new monoclonal antibodies identify distinct onco-foetal antigens on undifferentiated ectoblastic cells. These antigens are developmentally regulated and absent from normal adult tissues, unlike other stage-specific antigens.

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    Area of Science:

    • Developmental Biology
    • Immunology
    • Cancer Research

    Background:

    • Onco-foetal antigens are expressed during development and in cancer.
    • Understanding their regulation is crucial for cancer diagnostics and therapeutics.
    • Previous studies identified stage-specific antigens like 75.12 on embryonal carcinoma cells.

    Purpose of the Study:

    • To characterize novel onco-foetal antigens.
    • To investigate their expression patterns in developmental and cancerous cells.
    • To differentiate these antigens from known stage-specific markers.

    Main Methods:

    • Generation of monoclonal antibodies (5.1.H, 8.7.D, 13.7.A) against Tera 1 membrane fractions.
    • Analysis of antigen expression on Tera 2 clone 13 cells and other cell types.

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  • Comparison with known differentiation markers (75.12, SSEA-1).

    • Main Results:

    • Monoclonal antibodies recognized distinct, developmentally regulated onco-foetal antigens.
    • Antigens were expressed on undifferentiated ectoblastic cells and fetal intestine-like structures.
    • These antigens were absent in normal adult tissues.
    • No role for fucosyltransferases or lectin-like structures in regulating 75.12 or SSEA-1 was found.

    Conclusions:

    • Novel onco-foetal antigens with restricted expression patterns have been identified.
    • These antigens represent potential biomarkers for undifferentiated ectoblastic cells.
    • The findings distinguish these antigens from broadly expressed stage-specific markers.