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TULP3 Regulates Proliferation and Differentiation of 3T3-L1 Preadipocytes Through the Hedgehog Signaling Pathway
Xinlin Jin1, Yu Zhang1, Yunzhou Wang2
1Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Abstract:
The TULP family was first identified in progressively obese mice, and TULP3, as a member of its family, has been much studied in tumor cells, but studies on its role in adipocytes have not yet been reported. This study found that the expression of TULP3 showed an increasing trend in the differentiation of 3T3-L1 cells, and overexpression of TULP3 enhanced the proliferation and differentiation capacity of the cells, while inhibition caused the opposite result. TULP3 is a negative regulator of the Hedgehog signaling pathway, which can control lipid metabolism in adipose tissues, but whether TULP3 can play a role in adipose tissues through the Hedgehog signaling pathway is not yet known. It was experimentally found that TULP3 could promote adipogenic differentiation of precursor adipocytes by inhibiting the activity of the Hedgehog signaling pathway. Our results elucidate the role of TULP3 in the generation of precursor adipocytes and provide useful information for a deeper understanding of the molecular mechanisms of adipocytogenesis, which will contribute to the improvement of the treatment of adipose tissue dysfunction or uncontrolled adipogenesis-related diseases.
Insights
Tubulin polymerization-depolymerizing protein 3 (TULP3) promotes adipocyte differentiation by inhibiting the Hedgehog signaling pathway. This finding offers insights into treating adipose tissue dysfunction and related diseases.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The TULP family, including TULP3, is known from obese mice studies and tumor cell research.
- The specific role of TULP3 in adipocytes and its mechanism of action in adipose tissue remain largely unexplored.
Purpose of the Study:
- To investigate the role and mechanism of TULP3 in the differentiation of adipocytes.
- To determine if TULP3 influences adipogenesis via the Hedgehog signaling pathway.
Main Methods:
- Utilized 3T3-L1 cells to study TULP3 expression during adipocyte differentiation.
- Manipulated TULP3 expression (overexpression and inhibition) to assess its impact on cell proliferation and differentiation.
- Investigated the interaction between TULP3 and the Hedgehog signaling pathway in adipogenesis.
Main Results:
- TULP3 expression increased during 3T3-L1 cell differentiation.
- Overexpression of TULP3 enhanced adipocyte proliferation and differentiation, while inhibition had the opposite effect.
- TULP3 was identified as a negative regulator of the Hedgehog signaling pathway, promoting adipogenic differentiation by inhibiting pathway activity.
Conclusions:
- TULP3 plays a crucial role in promoting adipogenic differentiation of precursor adipocytes.
- Inhibition of the Hedgehog signaling pathway by TULP3 is a key mechanism in adipogenesis.
- These findings provide valuable information for understanding adipocytogenesis and developing treatments for adipose tissue dysfunction and related diseases.