Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
Types of RNA01:20

Types of RNA

5.5K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.5K
RNA Interference01:23

RNA Interference

25.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.8K
Nucleic Acid Structure01:25

Nucleic Acid Structure

5.8K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
5.8K
Nucleic Acids02:43

Nucleic Acids

43.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.0K
MicroRNAs01:22

MicroRNAs

21.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-Binding Protein Occupancy Composition Predicts Long Noncoding RNA Subcellular Localization.

International journal of molecular sciences·2026
Same author

Differential regulation of long noncoding RNAs by endogenous and exogenous reactive oxygen species-generating prooxidants in NIH3T3 cells.

PloS one·2025
Same author

Metabolic labeling of RNA using ribonucleoside analogs enables the evaluation of RNA synthesis and degradation rates.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2024
Same author

Recent Advances and Prospects in RNA Drug Development.

International journal of molecular sciences·2024
Same author

Identification of Two Long Noncoding RNAs, Kcnq1ot1 and Rmst, as Biomarkers in Chronic Liver Diseases in Mice.

International journal of molecular sciences·2024
Same author

Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis.

PloS one·2017

Related Experiment Video

Updated: May 10, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.3K

Biomolecules Interacting with Long Noncoding RNAs.

Hidenori Tani1

  • 1Department of Health Pharmacy, Yokohama University of Pharmacy, 601 Matano, Totsuka, Yokohama 245-0066, Japan.

Biology
|April 26, 2025
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) are key regulators of gene expression, interacting with DNA and RNA to control cellular functions. This review integrates structural, functional, and mechanistic insights into lncRNA interactions for potential therapeutic applications.

Keywords:
RNA–DNA triplexescellular functiongene regulationlong noncoding RNAmiRNA sponge

More Related Videos

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.5K
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.1K

Related Experiment Videos

Last Updated: May 10, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
07:24

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

2.3K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.5K
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Long noncoding RNAs (lncRNAs) are RNA transcripts >200 nucleotides that do not encode proteins.
  • lncRNAs play crucial roles in diverse biological processes, including embryogenesis and pathogenesis.
  • Their functions are mediated through interactions with DNA, RNA, and proteins.

Purpose of the Study:

  • To provide a comprehensive review of lncRNA interactions with various biomolecules.
  • To integrate structural, functional, and mechanistic perspectives on lncRNA biology.
  • To discuss emerging roles in development and disease, and evaluate diagnostic/therapeutic potential.

Main Methods:

  • Literature review and synthesis of existing research on lncRNA interactions.
  • Analysis of mechanisms including DNA triplex formation, chromatin organization, and RNA processing modulation.
  • Evaluation of methodologies for studying lncRNA interactions.

Main Results:

  • lncRNAs interact with DNA to influence chromatin structure and gene expression.
  • lncRNAs regulate RNA-based processes such as mRNA stability, translation, and splicing.
  • lncRNAs exhibit structural versatility enabling diverse biomolecular interactions.

Conclusions:

  • lncRNA interactions are central to intricate regulatory networks governing cellular processes.
  • Understanding lncRNA interactions offers insights into molecular biology and disease mechanisms.
  • lncRNAs hold significant potential for future diagnostics and therapeutics.