Genome-Wide Identification of the BREVIS RADIX Gene Family in Foxtail Millet: Function, Evolution, and Expression

Foxtail millet (Setaria italica), domesticated from green foxtail (Setaria viridis), is crucial for global food security. Given increasing environmental challenges, exploring its stress-resistance mechanisms via researching the BREVIS RADIX (BRX) gene family is urgent.

The study combines advanced bioinformatics and experimental validation. It uses phylogenetic, motif, domain, synteny analyses, miRNA prediction, and quantitative expression profiling under stress.

Phylogenetic analysis reveals new sub-clades and trajectories. Motif and domain analyses find new conserved elements. Statistical models show unique selective forces. Synteny analysis identifies genomic architecture and new blocks. miRNA prediction reveals gene-miRNA interactions, and expression profiling shows new patterns.

The research offers new insights into the BRX family's role in foxtail millet's growth and stress responses, laying a foundation for crop genetic improvement and enhancing stress resilience for global food security.