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MicroRNAs in Diffuse Large B-Cell Lymphoma (DLBCL): Biomarkers with Prognostic Potential.
Yuliya A Veryaskina1,2, Sergei E Titov1,3, Igor B Kovynev4
1Laboratory of Molecular Genetics, Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk 630090, Russia.
Cancers
|April 26, 2025
Summary
This study identified specific microRNAs (miRNAs) as potential biomarkers for diffuse large B-cell lymphoma (DLBCL) prognosis. Upregulated miRNA-23a, miRNA-125a, and miRNA-100 indicate better survival in DLBCL patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Diffuse large B-cell lymphoma (DLBCL) exhibits heterogeneity influencing tumor growth, treatment response, and prognosis.
- Genetic and epigenetic factors contribute to DLBCL's complex nature.
- Accurate prognostic markers are crucial for optimizing DLBCL patient management.
Purpose of the Study:
- To identify microRNAs (miRNAs) that can enhance prognostic accuracy in diffuse large B-cell lymphoma (DLBCL) patients.
- To investigate the role of specific miRNAs in predicting DLBCL patient outcomes.
Main Methods:
- MicroRNA sequencing was performed on bone marrow (BM) samples from DLBCL patients.
- Real-time RT-PCR was used to analyze miRNA expression levels in lymph node (LN) and BM samples.
- Kaplan-Meier survival analysis was employed to correlate miRNA expression with patient survival.
Main Results:
- Reduced expression of several miRNAs (miRNA-10b, -100, -125a, -125b, -126, -143, -23a, let-7a) was observed in DLBCL patients with poor prognosis.
- Upregulated miRNA-23a, miRNA-125a, and miRNA-100 correlated with better overall survival.
- Elevated levels of miRNA-151a, miRNA-148b, miRNA-192, PD-L1, TIMP1, TOP2A, and TP53 were detected in DLBCL BM samples compared to non-cancerous controls.
Conclusions:
- miRNA-23a, miRNA-125a, and miRNA-100 are potential prognostic biomarkers for DLBCL.
- Altered expression of specific miRNAs and genes (miRNA-151a, miRNA-148b, miRNA-192, PD-L1, TIMP1, TOP2A, TP53) indicates bone marrow involvement in DLBCL, even without clear pathological signs.
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