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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: May 10, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Advances in Microfluidic Single-Cell RNA Sequencing and Spatial Transcriptomics.

Yueqiu Sun^1,2, Nianzuo Yu^1,2, Junhu Zhang^1,2

¹State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130000, China.

|April 26, 2025

Summary

This summary is machine-generated.

Microfluidic chips enhance single-cell and spatial multi-omics analysis by enabling high-throughput screening. These advanced technologies offer new insights into tissue gene expression, homeostasis, and disease mechanisms.

Keywords:

microfluidics single cell single-cell RNA sequencing (scRNA-seq)spatial transcriptome

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Last Updated: May 10, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Published on: July 6, 2022

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Micro- and nano-fabrication technologies have significantly advanced single-cell and spatial omics.
Microfluidic chips offer integration and compartmentalization for high-throughput parallel reactions.
Omics technologies are increasingly integrated with microfluidics for molecular characterization.

Purpose of the Study:

To survey microfluidic methods for single-cell and spatial multi-omics.
To assess the advantages and limitations of current microfluidic approaches.
To highlight advancements in microfluidic-based multi-omics over the past decade.

Main Methods:

Review of microfluidic chip applications in single-cell and spatial multi-omics.
Analysis of integration of transcriptomics technologies with microfluidics.
Assessment of microfluidic adaptations for multi-omics insights.

Main Results:

Microfluidic chips facilitate high-throughput single-cell screening and analysis.
Integration of microfluidics with omics technologies provides molecular insights into tissue gene expression.
Microfluidic methods reveal static and dynamic processes in tissue homeostasis and disease.

Conclusions:

Microfluidics has greatly improved single-cell and spatial multi-omics.
These methods offer new insights into development, neuroscience, and disease mechanisms.
Future perspectives include advances in translational and clinical medicine.