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Updated: May 10, 2025
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Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging
Published on: April 19, 2024
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Dynamic Neutrophil Subsets and Function in Lung Transplant Recipients: Insights from a One-Year Longitudinal Pilot
Naomi Kaisar-Iluz1, Merav E Shaul1, Ofir Deri2
1Hadassah Medical Center, Institute of Pulmonary Medicine, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112102, Israel.
Journal of Clinical Medicine
|April 26, 2025
Summary
Neutrophil subsets change after lung transplantation, with increased low-density neutrophils (LDNs) and altered normal-density neutrophil (NDN) function. These dynamic neutrophil changes offer potential biomarkers for lung transplant recipients.
Area of Science:
- Immunology
- Transplantation Biology
Background:
- Neutrophils are critical innate immune cells with identified subpopulations: normal-density neutrophils (NDNs) and low-density neutrophils (LDNs).
- LDNs are associated with chronic inflammation and cancer, while neutrophils play significant roles in lung transplant (LTx) complications like rejection and dysfunction.
Purpose of the Study:
- To investigate dynamic changes in neutrophil subsets and function in lung transplant recipients during the first year post-transplant.
- To explore the clinical relevance of neutrophil plasticity in LTx.
Main Methods:
- Blood samples were collected from 11 LTx recipients at multiple time points.
- Low-density neutrophils (LDNs) and normal-density neutrophils (NDNs) were isolated for analysis.
- Reactive oxygen species (ROS) production by NDNs was measured, and neutrophil phenotypic markers were analyzed via flow cytometry.
Main Results:
- The ratio of LDNs to NDNs increased at 3 and 6 months post-LTx.
- Expression of markers like CD62-L, PDL-1, CD15, and CXCR4 on neutrophils was modulated.
- NDN reactive oxygen species (ROS) production showed transient changes, decreasing at 6 months before returning to baseline.
Conclusions:
- Neutrophils undergo significant dynamic alterations in the first year following lung transplantation.
- Understanding neutrophil plasticity may lead to novel biomarkers and therapeutic strategies for LTx patients.
