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TOTUM-854 Human Circulating Bioactives Preserve Endothelial Cell Function.

Fabien Wauquier1, Doriane Ripoche2, Line Boutin-Wittrant1

  • 1Clinic'n'Cell SAS, Faculté de Médecine, 28 Place Henri Dunant, 63001 Clermont-Ferrand, France.

Nutrients
|April 26, 2025
PubMed
Summary
This summary is machine-generated.

TOTUM-854, a plant extract, protects human endothelial cells from damage. Its metabolites, identified in human serum, reduce hypertension markers, suggesting vascular protection benefits.

Keywords:
clinical trialex vivohuman endothelial cellshuman metaboliteshypertensionlipotoxic stressoxidative stressplant extract

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Area of Science:

  • Cardiovascular Research
  • Pharmacology
  • Cell Biology

Background:

  • TOTUM-854 is a patented plant extract blend with potential antihypertensive properties.
  • Existing literature lacks detailed cellular mechanisms of action, especially in humans.
  • Further research is needed to optimize therapeutic strategies for hypertension prevention.

Purpose of the Study:

  • To investigate the cellular mechanisms of TOTUM-854 in preventing hypertension.
  • To evaluate the protective effects of TOTUM-854 metabolites on human endothelial cells.
  • To explore the role of these metabolites in vascular protection.

Main Methods:

  • Demonstrated TOTUM-854 efficacy in an L-NAME mouse model for hypertension prevention.
  • Collected human serum from volunteers before and after TOTUM-854 intake to identify circulating metabolites via UPLC-MS.
  • Incubated human umbilical vein endothelial cells (HUVECs) with serum containing metabolites, with and without palmitate challenge.

Main Results:

  • TOTUM-854 metabolites significantly decreased angiotensin-converting enzyme 1 (ACE-1) activity by 32%.
  • Reduced oxidative stress markers, including reactive oxygen species (ROS) and Nox2 gene expression.
  • Inhibited inflammatory responses, decreasing Interleukin-1 beta (IL-1β) release and MCP-1/VCAM-1 gene expression.

Conclusions:

  • TOTUM-854 demonstrates a protective role in human endothelial cells.
  • The study highlights the efficacy of an ex vivo clinical approach using circulating metabolites.
  • Findings support the contribution of TOTUM-854 metabolites to human vascular protection.