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Related Experiment Video

Updated: May 10, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

A Multi-Modal Light Sheet Microscope for High-Resolution 3D Tomographic Imaging with Enhanced Raman Scattering and

Pooja Kumari¹, Björn Van Marwick¹, Johann Kern²

¹CeMOS Research and Transfer Center, Mannheim University of Applied Sciences, 68163 Mannheim, Germany.

Sensors (Basel, Switzerland)

|April 26, 2025

Summary

This summary is machine-generated.

We developed a new multi-modal Raman light sheet microscope for advanced 3D imaging of cellular models. This label-free system enhances molecular and structural details in complex biological specimens.

Keywords:

3D molecular imaging Deep Image Prior (DIP)fluorescence suppression light sheet microscopy multi-modal imaging raman tomography super-resolution

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Last Updated: May 10, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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Published on: August 16, 2012

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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Published on: October 8, 2013

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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Published on: August 15, 2014

Area of Science:

Biomedical Optics
Cellular Imaging
Biotechnology

Background:

Three-dimensional (3D) cellular models are crucial for histology, oncology, and tissue engineering.
Advanced imaging techniques are needed to study complex biological phenomena in these models.

Purpose of the Study:

To present a novel multi-modal Raman light sheet microscope for label-free, high-resolution 3D imaging.
To integrate optical and computational strategies for enhanced imaging of biomolecular and structural features.

Main Methods:

Developed a multi-modal microscope capturing elastic (Rayleigh), inelastic (Raman) scattering, and fluorescence signals.
Utilized a 532 nm excitation wavelength to enhance Raman scattering efficiency and suppress fluorescence.
Integrated a Deep Image Prior (DIP) algorithm for unsupervised denoising and resolution enhancement.

Main Results:

Achieved label-free, high-resolution tomographic imaging of 3D cellular models.
Demonstrated robust fluorescence suppression, avoiding drawbacks of near-infrared modalities.
Preserved critical molecular details and minimized artifacts through computational enhancement.

Conclusions:

The developed microscope offers advanced 3D imaging capabilities for complex biological specimens.
This synergy of optical and computational methods advances biomedical research, diagnostics, and therapeutics.