A LAMP Detection System Based on a Microfluidic Chip for Pyricularia grisea

Area of Science:

• Agricultural Science
• Biotechnology
• Plant Pathology

Background:

• Rice blast, caused by Pyricularia grisea, is a major global threat to rice yield and quality.
• Accurate and rapid detection of Pyricularia grisea is crucial for effective rice blast management.

Purpose of the Study:

• To develop an integrated, portable microfluidic detection system for rapid and sensitive identification of Pyricularia grisea.
• To improve detection efficiency and reduce reagent loss using microfluidic chip design.

Main Methods:

• Development of an integrated microfluidic system incorporating a microfluidic chip, temperature control module, and OpenMv camera.
• Utilized micro-mixing channels with shear structures for enhanced mixing (approx. 98%) and flow-blocking valves to minimize reagent loss.
• Employed the Loop-mediated Isothermal Amplification (LAMP) method for DNA detection at a stable temperature of 65 °C.

Main Results:

• The microfluidic system achieved a detection range of 10 copies/μL to 10^5 copies/μL for Pyricularia grisea.
• Detection was completed within 45 minutes, demonstrating rapid results.
• Specificity and interference experiments confirmed the high reliability and accuracy of the developed detection method.

Conclusions:

• The integrated microfluidic LAMP detection system provides a rapid, sensitive, and reliable method for Pyricularia grisea detection.
• This system holds significant potential for early and effective management of rice blast disease.
• The portable nature of the system facilitates on-site detection and disease control strategies.