A LAMP Detection System Based on a Microfluidic Chip for Pyricularia grisea

As one of the major rice fungal diseases, blast poses a serious threat to the yield and quality of rice globally. It is caused by the pathogen Pyricularia grisea. Therefore, the development of rapid, accurate, and portable microfluidic detection system for Pyricularia grisea is important for the control of rice blast. This study presents an integrated microfluidic detection system for the rapid and sensitive detection of Pyricularia grisea using the LAMP detection method. The microfluidic detection system includes a microfluidic chip, a temperature control module, and an OpenMv camera. The micro-mixing channels with shear structures improve the mixing efficiency to about 98%. Flow-blocking valves are used to reduce reagent loss in the reaction chamber. The temperature control module is used to heat the reaction chamber, maintaining a stable temperature of 65 °C. The microfluidic chip detection chamber is used for image inspection using an OpenMv camera. The developed system can detect Pyricularia grisea in the range of 10 copies/μL-10⁵ copies/μL within 45 min. Specificity and interference experiments were performed on Pyricularia grisea, validating the method's good reliability. This LAMP detection system based on a microfluidic chip has strong potential in the early and effective detection of rice blast.