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Two Methods for Detecting PCM Residues in Vegetables Based on Paper-Based Sensors
Jiazheng Sun1,2,3, Shiling Li1,2,3, Xijun Shao1,2,3
1School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China.
Sensors (Basel, Switzerland)
|April 26, 2025
Summary
A new immunoassay method uses gold and fluorescence-labeled antibodies to detect procymidone (PCM) fungicide residues in vegetables. This rapid, sensitive dual-test approach offers efficient screening and accurate quantification for food safety.
Area of Science:
- Agricultural Chemistry
- Analytical Chemistry
- Biotechnology
Background:
- Procymidone (PCM) is a widely used fungicide with increasing residues in vegetables.
- Accurate detection methods are crucial for ensuring food safety and managing fungicide use.
Purpose of the Study:
- To develop a sensitive and rapid immunoassay for detecting procymidone (PCM) residues in vegetables.
- To create a dual detection system combining gold and fluorescence-labeled antibodies for efficient screening and quantification.
Main Methods:
- Development of immunochromatographic strips using gold nanoparticle-labeled and fluorescent microsphere-labeled monoclonal antibodies (mAbs).
- Optimization of assay conditions to achieve high sensitivity and accuracy.
- Validation of the method using various vegetable matrices.
Main Results:
- Fluorescent strips achieved a limit of detection (LOD) of 1.67 ng/mL (quantitative) and 50 ng/mL (visual).
- Gold strips provided a visual detection range of 50 to 200 ng/g.
- High accuracy (94.98%-103.82%) and precision (CV 1.97%-8.91%) were demonstrated for both methods.
- The dual-method approach showed excellent performance in real vegetable samples.
Conclusions:
- The developed immunoassay method is sensitive, rapid, and accurate for detecting procymidone residues in vegetables.
- The dual-test strategy allows for efficient screening of negative samples and precise quantification of positive samples.
- This method has significant market potential for practical food safety applications.
