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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
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Related Experiment Video

Updated: May 10, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver
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Comprehensive circRNA expression profile and hub genes screening during human liver development.

Si Ying Li1, Shu Ting Liu1, Chen Yi Wang1

  • 1Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, China.

Annals of Medicine
|April 26, 2025
PubMed
Summary
This summary is machine-generated.

This study reveals circular RNA (circRNA) roles in human liver development, identifying key genes and pathways involved in embryonic organ development and potential adult liver diseases.

Keywords:
Human livercircular RNAsembryonic developmenthigh-throughput RNA sequencing

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Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing
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Area of Science:

  • Genomics
  • Developmental Biology
  • RNA Biology

Background:

  • Non-coding RNAs, specifically circular RNAs (circRNAs), are increasingly recognized for their roles in cellular processes.
  • Understanding circRNA expression during human liver development is crucial for insights into liver diseases.

Purpose of the Study:

  • To investigate the roles and regulatory networks of circRNAs in human liver development.
  • To identify key circRNAs and genes involved in embryonic liver formation.

Main Methods:

  • High-throughput sequencing and bioinformatics analysis of human fetal liver samples across developmental stages.
  • Differential expression analysis, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG), and Disease Ontology (DO) enrichment analyses.
  • Construction of co-expression and protein-protein interaction (PPI) networks, followed by validation using quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Main Results:

  • Identified 645 differentially expressed circRNAs and 5,145 differentially expressed mRNAs.
  • circRNA activity was significantly boosted during the hepatoblastic stage, involved in nervous system regulation, embryonic organ development, and digestive system development.
  • Key pathways implicated include PI3K-AKT, MAPK, and calcium signaling; three hub genes (LGR5, FOXL1, RSPO3) were identified as potentially crucial for liver development.

Conclusions:

  • This study provides the first comprehensive insights into circRNA roles in human liver development.
  • Established circRNA regulatory networks and identified key genes, laying the foundation for future research into molecular and signaling networks in liver development and disease.