Self-Assembled Multilayered Concentric Supraparticle Architecture

Area of Science:

• Nanotechnology and Materials Science
• Biomedical Engineering
• Drug Delivery Systems

Background:

• Supraparticles (SPs) are emerging as versatile platforms for catalysis, photonics, and medicine due to their unique properties.
• Synthesizing novel SPs with complex internal structures presents a significant challenge in materials science.
• Existing SPs often lack the intricate architectures required for advanced applications, particularly in nanomedicine.

Purpose of the Study:

• To introduce a novel class of Self-Assembled Multilayered Supraparticles (SAMS) with complex internal structures.
• To investigate the synergistic interactions between gold nanoparticles, lipidoid, and gelatin in SAMS formation.
• To evaluate the potential of SAMS as a platform for efficient in vivo delivery of therapeutic nucleic acids.

Main Methods:

• Fabrication of SAMS through a synergistic three-way interaction involving gold nanoparticles, lipidoid, and gelatin.
• Characterization of SAMS structure, including concentric lamellar spherical architecture, interlayer spacing (3.5 ± 0.2 nm), and diameter (156.8 ± 56.6 nm).
• Analysis of physical and chemical factors influencing SAMS formation, such as nanoparticle size, lipidoid chain length, and surface chemistry.

Main Results:

• Successful synthesis of SAMS with controlled multilayered spherical structures.
• Demonstration that SAMS formation is critically dependent on nanoparticle size, lipidoid chain length, and surface chemistry.
• Efficient in vivo delivery of labile payloads, including siRNA, achieving dose-dependent gene silencing, with potential for mRNA delivery.

Conclusions:

• This work introduces a novel supraparticle structure (SAMS) and a new interaction phenomenon for their formation.
• SAMS exhibit tunable properties influenced by physical and chemical factors, enabling precise control over interparticle interactions.
• SAMS demonstrate significant potential as an advanced nanomedicine platform for effective in vivo nucleic acid delivery.