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Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

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A Reversible and Dynamic Surface Functionalization for Fluidity Controlled Multivalent Recognition of Lectins and

Thomas Hix-Janssens¹, Adam Tillo¹, Hanna Isaieva¹

¹Biofilms Research Center for Biointerfaces, Department of Biomedical Science, Faculty of Health and Society, Malmö University, Malmö, 205 06, Sweden.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|April 26, 2025

Summary

Researchers designed reusable bacterial receptors using self-assembled monolayers. These mimic host cell ligands, effectively capturing bacteria like Pseudomonas aeruginosa by targeting its LecA adhesin.

Keywords:

bacterial recognition membrane mimic multivalent receptor rSAM rewritable surfaces

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

Biomaterials Science
Surface Chemistry
Microbiology

Background:

Bacterial infections, particularly from multidrug-resistant strains like Pseudomonas aeruginosa, pose significant health challenges.
Understanding bacterial adhesion mechanisms, such as the role of adhesins like LecA, is crucial for developing targeted interventions.
Mimicking natural ligand display on host cells is a promising strategy for creating novel antibacterial surfaces.

Purpose of the Study:

To design and characterize multivalent bacterial receptors using reversible self-assembled monolayers (rSAMs).
To investigate the binding affinity of these receptors for the Pseudomonas aeruginosa adhesin, LecA.
To demonstrate the ability of these functionalized surfaces to capture specific bacterial species.

Main Methods:

Fabrication of rSAMs on gold and glass substrates using binary amphiphile mixtures.
Decoration of rSAMs with β-galactose (Gal) or sialic acid (SA) ligands.
Incubation of functionalized surfaces with Pseudomonas aeruginosa (PA) and Streptococcus gordonii (SG) cultures.
Comparison of bacterial capture efficiency between mobile ligand displays (rSAMs) and static ligand surfaces.

Main Results:

rSAMs functionalized with Gal or SA demonstrated strong affinity for the bacterial adhesin LecA.
Surfaces with mobile ligands showed significantly higher bacterial capture compared to those with static ligands.
The rSAMs exhibited tunable ligand ratios, allowing for adaptable and reusable bacterial recognition surfaces.
Successful capture of PA and SG bacteria was demonstrated on the Gal and SA functionalized rSAMs.

Conclusions:

Reversible self-assembled monolayers offer a versatile platform for creating multivalent bacterial receptors.
Mobile ligand display on rSAMs enhances affinity and specificity for bacterial adhesins like LecA.
This wet chemical surface functionalization approach provides rapid restorability and adaptability for advanced biomaterial design.