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Updated: May 14, 2025

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Published on: June 28, 2013
Emerging structure of chlorovirus PBCV-1
James L Van Etten1, Irina V Agarkova1, David D Dunigan1
1Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA, 68583-0900.
Abstract:
The large plaque-forming chloroviruses infect isolates of eukaryotic chlorella-like green algae. Initial cryo-electron microscopy (cryo-EM) studies revealed that PBCV-1 was icosahedral, with a multilaminate shell surrounding an electron-dense core, and that PBCV-1 particles measured about 1900 Å in diameter with a triangulation number of 169d. However, as described in this review cryo-EM procedures have improved and PBCV-1 is more complex than originally described. A five-fold symmetry reconstruction of cryo-EM images at 8.5 Å revealed that the virus contains a unique vertex with a spike-structure and an internal single lipid bi-layered membrane. Improvement to 3.5 Å resolution revealed that the capsid contains 30 virus-encoded proteins and that it contains six different types of capsomers. The outer surface of three of the six types of capsomers are attached to fiber structures.
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