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Updated: May 10, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Identification of mitophagy-related genes in patients with acute myocardial infarction
Ju-Ying Li1,2, Hong-Kui Chen2, Yi-Hao Huang3
1The First People's Hospital of Yibin, Yibin, Sichuan Province, 644000, China.
Abstract:
Mitophagy is involved in acute myocardial infarction (AMI) process. However, the role of mitophagy-related genes (MRGs) in the AMI process is not well illustrated. We identified MRGs involved in AMI by bioinformatics analysis. The external datasets were employed for the validation of the MRGs, alongside the execution of cellular and animal experiments. Forty-five MRGs were detected, and machine learning identified the top four hub genes, namely ALDH2, ACSL1, IL1B, and GABARAPL1. Additionally, an external validation set was used to screen for three diagnostic markers (ACSL1, IL1B, and GABARAPL1) among these hub genes. Immune infiltration analysis revealed changes in the immune microenvironment among patients with AMI. Finally, the significant upregulation of ACSL1, IL1B, and GABARAPL1 in both cellular and animal models was confirmed. The occurrence of mitophagy was observed in the cell model through transmission electron microscopy (TEM). Our study demonstrated that ACSL1, IL1B, and GABARAPL1 possess potential biomarkers for AMI.
Insights
This study identifies key mitophagy-related genes (MRGs) involved in acute myocardial infarction (AMI). ACSL1, IL1B, and GABARAPL1 show potential as diagnostic biomarkers for AMI.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Cellular Biology
Background:
- Mitophagy plays a role in acute myocardial infarction (AMI).
- The specific mitophagy-related genes (MRGs) implicated in AMI pathogenesis require further elucidation.
- Understanding MRGs is crucial for developing diagnostic and therapeutic strategies for AMI.
Purpose of the Study:
- To identify and validate MRGs associated with AMI.
- To explore the diagnostic potential of identified MRGs in AMI.
- To investigate the role of mitophagy and specific MRGs in the AMI pathological process.
Main Methods:
- Bioinformatic analysis of publicly available datasets to identify MRGs in AMI.
- Machine learning algorithms to pinpoint hub genes among identified MRGs.
- External dataset validation, cellular experiments, animal models, and transmission electron microscopy (TEM) to confirm findings.
Main Results:
- Forty-five MRGs were identified, with ALDH2, ACSL1, IL1B, and GABARAPL1 identified as key hub genes.
- ACSL1, IL1B, and GABARAPL1 were validated as potential diagnostic markers for AMI using an external dataset.
- Significant upregulation of ACSL1, IL1B, and GABARAPL1 was confirmed in cellular and animal AMI models, with mitophagy observed via TEM.
Conclusions:
- ACSL1, IL1B, and GABARAPL1 are significantly upregulated in AMI and demonstrate potential as diagnostic biomarkers.
- The study highlights the role of specific MRGs in the intricate molecular mechanisms underlying AMI.
- Further research into these MRGs could pave the way for novel diagnostic tools and therapeutic interventions for AMI.
