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Updated: May 2, 2026

A Micropatterning Assay for Measuring Cell Chirality
Published on: March 11, 2022
ChiraKit: an online tool for the analysis of circular dichroism spectroscopy data
Osvaldo Burastero1,2, Nykola C Jones3, Lucas A Defelipe1,2
1European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
Abstract:
Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has been used to understand secondary structure, folding, and binding of proteins and nucleic acids. Here, we present ChiraKit, a user-friendly, online, and open-source tool to process raw CD data and perform advanced analysis. ChiraKit features include the calculation of protein secondary structure with the SELCON3 and SESCA algorithms, estimation of peptide helicity using the helix-ensemble model, the fitting of thermal/chemical unfolding or user-defined models, and the decomposition of spectra through singular value decomposition or principal component analysis. ChiraKit can be accessed at https://spc.embl-hamburg.de/.
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