Single-Cell Transcriptome Reveals the Cellular Response to PEG-Induced Stress in Wheat Leaves

Drought is a major factor limiting the production and yield of wheat bread (Triticum aestivum). Therefore, investigating the wheat drought-related response mechanism is an urgent priority. Here, the single-cell transcriptome of drought-nonsusceptible and susceptible wheat seedlings subjected to PEG-induced stress was systematically analyzed to study the drought-related response at the cellular level. We identified five major cell types using known marker genes and constructed a wheat leaf cell atlas. On this foundation, several potential specific marker genes for each cell type were identified, which provide a reference for further cell type annotation. Moreover, we identified cellular heterogeneity in the drought-related response mechanisms and regulatory networks among cell types. Specifically, the drought response of mesophyll cells was correlated with the photosynthetic pathway. Pseudotime trajectory analysis revealed the transition of epidermal cells from their normal function to a defense response under stress. Moreover, we also characterized the genes associated with the drought response. Notably, we identified two transcription factors (TraesCS1D02G223600 and TraesCS1D02G072900) as master regulators in most cell types. Our study provides detailed insights into the response heterogeneity of cells under PEG-induced stress. The gene resources obtained in our study could be applied to breed better crop plants with improved drought tolerance.